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KMID : 0380020220370020071
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Korean Journal of Biotechnology and Bioengineering
2022 Volume.37 No. 2 p.71 ~ p.81
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Development of an Antibody Probe for Specific Detection of Ammonia-Oxidizing Archaea
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Chu Yeon-Jin
Cheong Dae-Eun
Kim Geun-Joong
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Abstract
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Ammonia oxidation is the first step of nitrification and plays an important role in global nitrogen cycle. Over the past 20 years, it has been founded that archaeal amoA gene is more abundant than bacterial amoA gene in diverse habitats. However, most of ammonia-oxidizing archaea (AOA) discovered by molecular techniques so far have not been cultivated yet in pure state. Thus, alternative methods of probing AOA are urgently needed. In this study, we attempted to produce antibody probes that specifically bound to a marine AOA strain, by generating polyclonal antibody using a subunit of specific ammonia monooxygenase (AMO) for ammonia oxidation. The resulting antibody could be used for detection of the AOA strain as illustrated by immunofluorescence staining in this work. Our results thus indicate that the typically generated polyclonal antibody would have broad in-vitro and in-situ applications in environmental microbiology and biotechnology.
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KEYWORD
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ammonia-oxidizing archaea, ammonia monooxygenase, polyclonal antibody, immunofluorescence
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